The Secondary Resistome of Methicillin-Resistant Staphylococcus aureus to β-Lactam Antibiotics.

ABSTRACT: Background: Therapeutic strategies for methicillin-resistant Staphylococcus aureus (MRSA) are increasingly limited due to the ability of the pathogen to evade conventional treatments such as vancomycin and daptomycin. This challenge has shifted the focus towards novel strategies, including the resensitization of β-lactams, which are still used as first-line treatments for methicillin-susceptible Staphylococcus aureus (MSSA). To achieve this, it is essential to identify the secondary resistome associated with the clinically relevant β-lactam antibiotics. Methods: Transposon-Directed Insertion Site Sequencing (TraDIS) was employed to assess conditional essentiality by analyzing the depletion of mutants from a highly saturated transposon library of MRSA USA300 JE2

SUBMITTER: Abdelmalek N 

PROVIDER: S-EPMC11851648 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature