Automated Engineering Protein Dynamics via Loop Grafting: Improving Renilla Luciferase Catalysis.

ABSTRACT: Engineering protein dynamics is a challenging and unsolved problem in protein design. Loop transplantation or loop grafting has been previously employed to transfer dynamic properties between proteins. We recently released a LoopGrafter Web server to execute the loop grafting task, employing eight computational tools and one database. The LoopGrafter method relies on the prediction of the local dynamic behavior of the elements to be transplanted and has successfully reconstructed previously engineered sequences. However, it was unclear whether catalytically competitive previously uncharacterized designs could be obtained by this method. Here, we address this question, showing how LoopGrafter generates viable loop-grafted chimeras of luciferases, how these chimeras encompass the activity of

SUBMITTER: Planas-Iglesias J 

PROVIDER: S-EPMC11851775 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature