Development of a Widely Accessible, Advanced Large-Scale Microfluidic Airway-on-Chip.

ABSTRACT: On-chip microfluidics are advanced in vitro models that simulate lung tissue's native 3D environment more closely than static 2D models to investigate the complex lung architecture and multifactorial processes that lead to pulmonary disease. Current microfluidic systems can be restrictive in the quantities of biological sample that can be retrieved from a single micro-channel, such as RNA, protein, and supernatant. Here, we describe a newly developed large-scale airway-on-chip model that employs a surface area for a cell culture wider than that in currently available systems. This enables the collection of samples comparable in volume to traditional cell culture systems, making the device applicable to any workflow utilizing these static systems (RNA isolation, ELISA, etc.). With our const

SUBMITTER: Rae B 

PROVIDER: S-EPMC11851814 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature