The viscoelastic properties of Nicotiana tabacum BY-2 suspension cell lines adapted to high osmolarity.

ABSTRACT: To survive and grow, plant cells must regulate the properties of their cellular microenvironment in response to ever changing external factors. How the biomechanical balance across the cell's internal structures is established and maintained during environmental variations remains a nurturing question. To provide insight into this issue we used two micro-mechanical imaging techniques, namely Brillouin light scattering and BODIPY-based molecular rotors Fluorescence Lifetime Imaging, to study Nicotiana tabacum suspension BY-2 cells long-term adapted to high concentrations of NaCl and mannitol. The molecular crowding in cytoplasm and vacuoles was examined, as well as tension in plasma membrane. To understand how sudden changes in osmolarity affect cellular mechanics, the response of the contr

SUBMITTER: Skrzypczak T 

PROVIDER: S-EPMC11852555 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature