Role of mouse adenovirus type 1 E4orf6-induced degradation of protein kinase R in pathogenesis.

ABSTRACT: Protein kinase R (PKR) is an interferon-induced antiviral protein activated by autophosphorylation in response to double strand DNA (dsRNA) and other stimuli. Activated PKR causes translation inhibition and apoptosis, and it contributes to proinflammatory responses, cell growth, and differentiation. Mouse adenovirus type 1 (MAV-1) counteracts PKR by causing its degradation via a viral protein, early region 4 open reading frame 6 (E4orf6). Degradation is dependent on E4orf6 binding to Cullin 2, a component of the MAV-1 E4orf6 ubiquitin ligase. We investigated the importance of E4orf6 for induction of PKR degradation by exploiting the ability to infect the natural host with the adenovirus MAV-1. First, we used a new PKR-deficient mouse strain, PKR-TKO. PKR-TKO mouse embryo fibroblasts (MEFs)

SUBMITTER: Edwards Iii DF 

PROVIDER: S-EPMC11852748 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature