Essential role of <i>cis</i>-encoded mature NS3 in the genome packaging of classical swine fever virus.

ABSTRACT: Classical swine fever virus (CSFV) is a member of the genus Pestivirus within the family Flaviviridae. The enveloped particles contain a plus-stranded RNA genome encoding a single large polyprotein. The processing of this polyprotein undergoes dynamic changes throughout the infection cycle. The release of mature NS3 from the polyprotein is mediated and regulated by the NS2 autoprotease and a cellular co-factor, restricting efficient cleavage to the early phases of infection. NS3 is a multifunctional viral enzyme exhibiting helicase, NTPase, and protease activities pivotal for viral replication. Hence, the release of mature NS3 fuels replication, whereas unprocessed NS2-3 precursors are vital for progeny virus production in later phases of infection. Thus far, no packaging sig

SUBMITTER: Lamp B 

PROVIDER: S-EPMC11852850 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature