Single-cell data-driven design of armed oncolytic virus to boost cooperative innate-adaptive immunity against cancer.

ABSTRACT: Oncolytic viruses have been considered promising cancer immunotherapies. However, oncovirotherapy agents impart durable responses in only a subset of cancer patients. Thus, exploring the cellular and molecular mechanisms underlying the heterogeneous responses in patients can provide guidance to develop more effective oncolytic virus therapies. Single-cell RNA sequencing (scRNA-seq) analysis of tumors responsive and non-responsive to oncovirotherapy revealed signatures of the tumor immune microenvironment associated with immune response. Thus, we designed and constructed an armed oncolytic virus, OV-5A, that expressed five genes with non-redundant functions. OV-5A treatment exhibits robust immune response against various tumors in multiple mouse models, peripheral blood mononuclear cell -pa

SUBMITTER: Zhao J 

PROVIDER: S-EPMC11852947 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature