The Dose-Dependent Effects of Fluorocitrate on the Metabolism and Activity of Astrocytes and Neurons.

ABSTRACT:

Background

Fluorocitrate (FC) ranging from 5 μM to 5 mM is often used as a specific metabolic inhibitor of the astrocytes to study astrocytic functions. Whether FC at such concentrations may affect neuronal metabolism and function in vivo remains unclear.

Methods

We examined the effects of FC on the ATP levels and Ca²⁺ activity of the astrocytes and neurons in the motor cortices of living mice using two-photon microscopy.

Results

We found that 25 μM and 250 μM of FC decreased the intracellular ATP levels and Ca²⁺ activity in the astrocytes in the motor cortex. Equally, 250 μM of FC, but not 25 μM of FC, reduced the intracellular ATP levels in the dendritic processes of the layer 5 pyramidal neurons. However, 25 μM of FC increased the neuro

SUBMITTER: Zhuang H 

PROVIDER: S-EPMC11853058 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature