Ultra-fast Cycling for Multiplexed Cellular Fluorescence Imaging.

ABSTRACT: Rapid analysis of single and scant cell populations is essential in modern diagnostics, yet existing methods are often limited and slow. Herein, we describe an ultra-fast, highly efficient cycling method for the analysis of single cells based on unique linkers for tetrazine (Tz)/trans-cyclooctene (TCO)-mediated quenching. Surprisingly, the quenching reaction rates were more than 3 orders of magnitude faster (t_1/2 <1 s) than predicted. This allowed multi-cycle staining and immune cell profiling within an hour, leveraging the accelerated kinetics to open new diagnostic possibilities for rapid cellular analyses.

SUBMITTER: Ko J 

PROVIDER: S-EPMC11853113 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature