Equivalent Circuit Modeling and Analysis for Microfluidic Electrical Impedance Monitoring of Single-Cell Growth.

ABSTRACT: Microfluidics has significantly advanced the field of single-cell analysis, particularly in studies related to cell growth, division, and heterogeneity. Electrical impedance spectroscopy (EIS), a label-free and non-invasive biosensing technique, has been integrated into microfluidic devices for high-throughput and long-term monitoring of single budding yeast cells. Accurate interpretation of EIS measurements of cell growth dynamics necessitates the establishment of theoretical equivalent circuit models for the single-cell sensing system. Here, we report on the development of equivalent circuit models of an in situ EIS sensing system to elucidate cell growth. Firstly, finite element modeling and simulation of an EIS measurement of cell growth in the EIS sensing unit were performed, guiding

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11853229 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature