Detection of Possible Resistance Mechanisms in Uropathogenic <i>Escherichia coli</i> Strains Isolated from Kidney Transplant Recipients Based on Whole Genome Sequencing.

ABSTRACT:

Background

Urinary tract infections are a global health concern, with uropathogenic Escherichia coli (UPEC) accounting for 80-90% of cases. Given the rise in antimicrobial resistance, our aim was to elucidate the genetic mechanisms behind low-level resistance to ciprofloxacin and fosfomycin (LLCR and LLFR) in UPEC strains, using whole-genome sequencing (WGS) to identify point mutations in chromosomal and plasmid genes.

Methods

A cohort UPEC was collected from kidney transplant recipients at the Virgen del Rocío University Hospital, Spain. Minimum inhibitory concentrations were determined for ciprofloxacin and fosfomycin to categorize strains into LLCR and LLFR. Twenty strains were selected for WGS, with genome annotations. Point mutations were identified and analyzed

SUBMITTER: Herrera-Espejo S 

PROVIDER: S-EPMC11853403 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature