Distinct Phenotypic and Molecular Characteristics of CD34^- and CD34⁺ Hematopoietic Stem/Progenitor Cell Subsets in Cord Blood and Bone Marrow Samples: Implications for Clinical Applications.

ABSTRACT: Background/Objectives: We aimed to identify the molecular signatures of primitive CD34⁺ and CD34^- hematopoietic stem/progenitor cell (HSC/HPC) subsets in cord blood and bone marrow samples. Methods: CD34⁺ and CD34^- HSC/HPC subsets from cord blood and bone marrow were characterized using flow cytometry, real-time PCR, and proteomic analysis to evaluate their phenotypic and molecular profiles. Results: Our findings revealed a significantly higher percentage of Lin^-CD34^-CD38^Low/- (-/-) cells than of Lin^-CD34⁺CD38^Low/- (+/-) cells in cord blood. Aldehyde dehydrogenase levels were significantly lower in (-/-) than in (+/-) cells. Clonogenic ability was lower in (-/-

SUBMITTER: Gaafar A 

PROVIDER: S-EPMC11853955 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature