Bioassay-Guide Preparative Separation of Hypoglycemic Components from Gynura divaricata (L.) DC by Conventional and pH-Zone Refining Countercurrent Chromatography.

ABSTRACT: Gynura divaricata (L.) DC is a long-used medicinal and edible plant in China folk. Its hyperglycemic effects have garnered increasing public attention in recent years. This study revealed that the ethyl acetate (EtOAc) and butanol (BuOH) partition fractions of G. divaricata crude extract exhibited significantly higher α-glucosidase inhibition activity and enhanced glucose uptake ability compared to other fractions. Guided by the hypoglycemic bioassay, these two fractions were subjected to isolation of active compounds using high-speed countercurrent chromatography (HSCCC). A two-phase solvent system composed of hexane-methyl tert-butyl ether (MtBE)-methanol-0.1% TFA water was employed for the separation of the EtOAc fraction by conventional HSCCC through a gradient elution st

SUBMITTER: Shen Z 

PROVIDER: S-EPMC11854274 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature