12/15-Lipoxygenase-Derived Electrophilic Lipid Modifications in Phagocytic Macrophages.

ABSTRACT: Macrophages remove apoptotic cells via phagocytosis, also known as efferocytosis, during inflammation to maintain tissue homeostasis. This process is accompanied by various metabolic changes in macrophages including the production of lipid metabolites by fatty acid oxygenases. Among these, highly reactive metabolites, called lipid-derived electrophiles (LDEs), modify cysteines and other nucleophilic amino acids in intracellular proteins. However, the landscape and functions of the modifications by these electrophilic metabolites have been poorly characterized. In this study, we used activity-based protein profiling to quantitatively profile the cysteine reactivity landscape and identify the potential targets of endogenous LDE modification during efferocytosis in mouse peritoneal macrophage

SUBMITTER: Deng K 

PROVIDER: S-EPMC11854370 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature