Immobilization Techniques for Food-Grade Nuclease P1 and Their Application in Nucleotide Production.

ABSTRACT: Nuclease P1 (NP1) is critical for producing 5'-nucleotides, which are essential flavor enhancers in the food industry. Traditional use of free NP1 is hampered by poor reusability, high costs, and potential residual enzyme protein contamination, compromising product quality. This study introduces a novel immobilization technique using a cross-linking approach with food-grade ion exchange resin AER1 to create NP1@AER1-GA. This method achieves an enzyme activity of 51,015 U/g, with a notable immobilization yield of 67.7%. The immobilized NP1@AER1-GA exhibits significantly enhanced stability and catalytic efficiency while ensuring industrial compatibility and maintaining stringent safety standards. Under optimized conditions, NP1@AER1-GA demonstrates exceptional performance in 5'-nucleotide pr

SUBMITTER: Yin XY 

PROVIDER: S-EPMC11854549 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature