Assessment of the Anti-Listeria Effect of Citrus limon Peel Extract In Silico, In Vitro, and in Fermented Cow Milk During Cold Storage.

ABSTRACT: In this study, the antimicrobial effect of Citrus limon peel extract against Listeria monocytogenes was analyzed in silico, tested in vitro, and validated in fermented cow milk during cold storage. The in silico analysis revealed that 4,5-di-O-caffeoyquinic acid interacts with L. monocytogenes proteins involved in colonization and intracellular survival. The in vitro experiments demonstrated that the anti-Listeria activity of Citrus limon peel extract is primarily attributed to limonene and phenolic compounds. In fermented milk stored at 4 °C for 7 days, the addition of Citrus limon peel extract resulted in a 2 Log reduction of L. monocytogenes compared to the control. Using the Baranyi and Roberts model, a significant decrease in the maximu

SUBMITTER: Mkadem W 

PROVIDER: S-EPMC11854709 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature