Vacuolar (H⁺)-ATPase Genes Are Essential for Cuticle and Wing Development in Locusta migratoria.

ABSTRACT:

Background/objectives

Vacuolar (H⁺)-ATPases (V-ATPases) are crucial in several significant biological processes, including intracellular transport, endocytosis, autophagy and protein degradation. However, their role in the growth and development of insects remains largely unknown. This study aimed to explore the molecular and functional properties of V-ATPases in Locusta migratoria.

Methods

LmV-ATPase genes were identified based on the locust transcriptome database and bioinformatics analysis. Quantitative reverse-transcription polymerase chain reaction was used to assess the relative expression of LmV-ATPases in different tissues and developmental stages. RNA interference combined with hematoxylin-eosin staining and transmission electron micr

SUBMITTER: Liu X 

PROVIDER: S-EPMC11854941 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature