<i>MLH1</i> Methylation Status and Microsatellite Instability in Patients with Colorectal Cancer.

ABSTRACT: Background/Objectives: The purpose of the current study was to compare the methylation of five regions of the CpG island of MLH1 with the presence of microsatellite instability (MSI) in colorectal cancer (CRC) patients. Methods: The study analyzed 138 CRC tumor samples. DNA extraction was performed, followed by bisulfite conversion. MLH1 gene methylation was assessed by methylation-specific PCR (MS-PCR), and the resulting fragments were analyzed using polyacrylamide gels. MSI was evaluated using multiplex PCR, and the fragments were run through capillary electrophoresis. R studio (v4.4.1) and SPSS (v29.0) software were used for the statistical analysis, and values of p < 0.05 were considered statistically significant. Results: The study showed 75.4

SUBMITTER: Rico-Mendez MA 

PROVIDER: S-EPMC11854980 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature