Proteomics and Expression of HIF2α/BNIP3L Signaling in Yak Brains at Different Altitudes.

ABSTRACT: The yak, a unique inhabitant of low-oxygen environments, exhibits brain adaptability to hypoxic conditions. However, the impact of hypoxia on yak brain proteomics and the expression of the HIF2α/BNIP3L signaling pathway remains unexplored. This study utilized TMT-based proteomics analysis to identify differentially expressed proteins (DEPs) in the cerebral cortexes of 9-month-old yaks at high (n = 3) and low (n = 3) altitudes. Additionally, qRT-PCR, Western blot, immunohistochemistry, and immunofluorescence were used to analyze HIF2α, BNIP3L, Beclin1, LC3-II, and cleaved caspase-3 expression in various brain regions from both altitude groups. KEGG analysis revealed that the DEPs were mainly concentrated in the synthesis and metabolism, DNA replication, and repair pathways. Sp

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC11855096 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature