A Double-Gene Metabarcoding Approach for the Authentication of Shrimp Surimi-Based Products.

ABSTRACT:

Background/objectives

Shrimp surimi-based products (SSPs) are composed of minced shrimp meat and are highly susceptible to food fraud as fish surimi. This study employed a double-gene metabarcoding approach to authenticate SSPs sold on Chinese e-commerce platforms.

Methods

16S rRNA and 12S rRNA genes were amplified and sequenced from 24 SSPs. Mislabeling was evaluated based on the correspondence between the ingredients (only those of animal origin) reported on the products' labels and the molecular results.

Results

Overall, 87.50% of SSPs (21/24) were found to be mislabeled. The replacement of Penaeus vannamei with other shrimp species was particularly noteworthy. Interestingly, in some SSPs, the primary species detected in terms of sequence abundance were not

SUBMITTER: Hu J 

PROVIDER: S-EPMC11855189 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature