Optimizing duodenal tissue acquisition for mechanistic studies of duodenal ablation in type 2 diabetes.

ABSTRACT:

Background and study aims

Histological analysis of regular duodenal biopsies to study morphologic changes after duodenal ablation for type 2 diabetes (T2D) and metabolic syndrome is hampered by variability in tissue orientation. We designed an optimized tissue acquisition protocol using duodenal cold snare resections to create tissue microarrays (TMAs) and to allow for single-cell RNA sequencing (scRNA-seq).

Patients and methods

The open-label DIRECT study included patients undergoing an upper gastrointestinal interventional endoscopy for non-duodenal indications. All underwent one ot two single-piece duodenal cold snare resections. Endpoints were safety, adequate histological orientation of specimen and TMA, and tissue dissociation quality for scRNA-seq. The optimized tissu

SUBMITTER: Busch CBE 

PROVIDER: S-EPMC11855243 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature