Catalytic Serine Labeling in Nonaqueous, Acidic Media.

ABSTRACT: Chemoselective modification of alkyl alcohols (e. g., serine residues) on proteins has been a daunting challenge especially in aqueous media. Herein, we report chemical modification of alkyl alcohols in protein and cell lysate samples using carboxylic acid-based bioconjugation media. The acidic medium is not only useful to suppress reactivity of other nucleophiles in proteins, but the medium also serves as a potentially biomolecule-compatible solvent. The acid-catalyzed acylation strategy has a unique selectivity paradigm compared to the common active-serine-targeted method and would act as a new strategy for studying biological roles of serine residues.

SUBMITTER: Ishizawa S 

PROVIDER: S-EPMC11855257 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature