Col5a3 Likely Promotes Adipogenesis of 3T3-L1 Through Oxidative Phosphorylation.

ABSTRACT:

Background

A recent study reported that a deficiency of Col5a3 reduces dermal fat. However, the regulatory mechanism of the Col5a3 gene on adipose deposition remains unclear.

Methods

In this study, we assessed the effects of Col5a3 interference on the proliferation and differentiation of 3T3-L1 preadipocytes through CCK-8, EdU staining, cell cycle detection, RT-qPCR, Western blot, a triglyceride assay, and Oil Red O staining. RNA-seq was then performed on differentiated adipocytes to identify key differentially expressed genes (DEGs) and signaling pathways.

Results

Col5a3 interference significantly promoted the proliferation of 3T3-L1 cells but inhibited their differentiation. RNA-seq analysis identified 368 DEGs, with the most significant

SUBMITTER: Wen S 

PROVIDER: S-EPMC11855316 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature