FABP4 Mediates Inflammation to Regulate Endometrial Epithelial Cell Function During Early Gestation in Sheep.

ABSTRACT: The objective of this experiment was to understand the impact of fatty acid binding protein 4 (FABP4)-mediated inflammation on sheep endometrial epithelial cells (sEECs). To model the hormonal environment of sheep during pregnancy, estradiol (E₂), progesterone (P₄) and interferon-tau (IFN-τ) were used. The sEECs were then stimulated with different concentrations of lipopolysaccharide (LPS) to determine suitable concentrations for inducing inflammation, while FABP4 expression was inhibited using a FABP4-specific inhibitor. The expression of genes related to inflammation, cell apoptosis, cell proliferation, endometrial tolerance, and embryo development were detected by real-time quantitative PCR and Western blot. The cell proliferation ability was detected by CCK-8, and

SUBMITTER: Chen Y 

PROVIDER: S-EPMC11855372 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature