Modeling Lactococcus lactis using a genome-scale flux model.

ABSTRACT:

Background

Genome-scale flux models are useful tools to represent and analyze microbial metabolism. In this work we reconstructed the metabolic network of the lactic acid bacteria Lactococcus lactis and developed a genome-scale flux model able to simulate and analyze network capabilities and whole-cell function under aerobic and anaerobic continuous cultures. Flux balance analysis (FBA) and minimization of metabolic adjustment (MOMA) were used as modeling frameworks.

Results

The metabolic network was reconstructed using the annotated genome sequence from L. lactis ssp. lactis IL1403 together with physiological and biochemical information. The established network comprised a total of 621 reactions and 509 metabolites, representing the overall metabolism of L. lactis. Experime

SUBMITTER: Oliveira AP 

PROVIDER: S-EPMC1185544 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature