Developmental Expression Patterns of miRNA in <i>Mythimna separata</i> Walker (Lepidotera: Noctuidae).

ABSTRACT:

Background/objectives

miRNAs are a family of single-stranded non-coding RNAs that regulate gene expression by targeting messenger RNAs (mRNAs) for suppression, with an average length of 22 nt. The oriental armyworm, Mythimna separata Walker, is a pest insect with long-distance migratory capability, which causes severe loss of grains and pastures in Eastern Asia, Southeastern Asia, and Oceania. This study aims to elucidate the post-transcriptional regulatory mechanisms of miRNAs in the development of this pest.

Methods

We carried out small RNA sequencing on samples from eggs, third instar larvae, pre-pupae, pupae, and adults.

Results

A total of 400 miRNAs were identified, among which 40 were known and 360 were novel miRNAs. Dynamic trend analysis of miRNAs revea

SUBMITTER: Liu Y 

PROVIDER: S-EPMC11855462 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature