Enhancement of the Precision ID Mitochondrial DNA Whole Genome System for Challenging Unidentified Human Remains.

ABSTRACT:

Background

The Precision ID mitochondrial (mt) DNA Whole Genome system is a fully automated massively parallel sequencing (MPS) solution for the whole mitochondrial genome. While extremely sensitive, the Precision ID system is susceptible to inhibitors and microbial DNA that are often co-extracted from human remains.

Methods

DNA templates spiked with varying amounts of hematin, humic acid, and calcium, along with bones containing degraded and non-human DNA, were sequenced using the Precision ID system with and without the addition of bovine serum albumin (BSA).

Results

BSA added to the initial PCR reaction successfully improved the robustness of the Precision ID system while not negatively impacting the sequencing success of uninhibited samples. The success of BSA is

SUBMITTER: Canale LC 

PROVIDER: S-EPMC11855493 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature