Integrating Transcriptomics and Metabolomics to Comprehensively Analyze Phytohormone Regulatory Mechanisms in Rhododendron chrysanthum Pall. Under UV-B Radiation.

ABSTRACT: In order to fully elucidate the roles and systems of phytohormones in UV-B radiation (UV-B) leaves of the Rhododendron chrysanthum Pall. (R. chrysanthum), we conducted a comprehensive analysis of how R. chrysanthum protects itself against UV-B using transcriptomic and metabolomic data. Transcript and metabolite profiles were generated by a combination of deep sequencing and LC-MS/MS (liquid chromatography-tandem mass spectrometry), respectively. Combined with physiological and biochemical assays, we studied compound accumulation, biosynthesis and expression of signaling genes of seven hormones and the effects of hormones on plant photosynthesis. The findings indicate that during leaf defense against UV-B, photosynthesis declined, the photosynthetic system was impaired

SUBMITTER: Yu W 

PROVIDER: S-EPMC11855671 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature