Characterization of PRDM9 Multifunctionality in Yak Testes Through Protein Interaction Mapping.

ABSTRACT: Meiotic recombination is initiated by the formation of programmed DNA double-strand breaks during spermatogenesis. PRDM9 determines the localization of recombination hotspots by interacting with several protein complexes in mammals. The function of PRDM9 is not well understood during spermatogenesis in mice or yaks. In this study, we applied yeast two-hybrid assays combined with next-generation sequencing techniques to screen the complete set of PRDM9-interacting proteins and explore its novel functions in yak spermatogenesis. Our results showed that 267 PRDM9-interacting proteins were identified. The gene ontology (GO) analysis of the interacting proteins revealed that the GO terms were primarily associated with spermatogenesis, positive regulation of double-strand break repair via homolo

SUBMITTER: Wang G 

PROVIDER: S-EPMC11855755 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature