Type 1 Diabetes Risk Variants Reduce Beta Cell Function.

ABSTRACT:

Introduction

The variants rs10517086 and rs1534422 are predictive of type 1 diabetes mellitus (T1DM) development and poor residual β cell function within the first year of diagnosis. However, the mechanism by which risk is conferred is unknown. We explored the impact of both variants on β cell function in vitro and assessed their relationship with C-peptide in people with T1DM and type 2 diabetes mellitus (T2DM).

Methods

Using CRISPR/Cas9, the variants were introduced into a β cell line (BRIN-BD11) and a T cell line (Jurkat cells) from which the conditioned media was applied to otherwise healthy β cells to model the inflammatory environment associated with these variants.

Results

Both variants significantly reduced glucose-stimulated insulin secretion, increased produ

SUBMITTER: Ratajczak W 

PROVIDER: S-EPMC11855905 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature