Recombinant Humanized Collagen Enhances Secreted Protein Levels of Fibroblasts and Facilitates Rats' Skin Basement Membrane Reinforcement.

ABSTRACT: Collagen and its peptides exhibit remarkable antioxidant activity, superior biocompatibility, and water solubility, making them a significant research focus in skin care. Hence, the recombinant humanized collagen types I, III, and XVII complexed with niacinamide were developed to address damage in human foreskin fibroblasts (HFF-1) caused by ultraviolet radiation and to evaluate basement membrane proteins in a rat skin model. The Cell Counting Kit-8 (CCK-8) assay showed that higher concentrations of the complex increased the survival of damaged cells by approximately 10% and 22%, respectively, compared to the normal group after 16 and 48 h of treatment. Further biochemical analyses using ELISA and immunofluorescence (IF) confirmed that the complex enhanced the expression of collagen type I

SUBMITTER: Ye S 

PROVIDER: S-EPMC11856143 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature