Macroscopic Assembly of Materials with Engineered Bacterial Spores via Coiled-Coil Interaction.

ABSTRACT: Herein, we report macroscopic materials formed by the assembly of engineered bacterial spores. Spores were engineered by using a T7-driven expression system to display a high density of pH-responsive self-associating proteins on their surface. The engineered surface protein on the spore surface enabled pH-dependent binding at the protein level and enabled the assembly of granular materials. Mechanical properties remained largely constant with changing pH, but erosion stability was pH-dependent in a manner consistent with the pH-dependent interaction between the engineered surface proteins. Our finding utilizes synthetic biology for the design of macroscopic materials and illuminates the impact of coiled-coil interaction across various length scales.

SUBMITTER: Korbanka L 

PROVIDER: S-EPMC11856349 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature