Bridging the Gap Between Platforms: Comparing Grape Phylloxera Daktulosphaira vitifoliae (Fitch) Microsatellite Allele Size and DNA Sequence Variation.

ABSTRACT: Grape phylloxera, Daktulosphaira vitifoliae (Fitch), is an economically significant pest of grapevines. Identification of phylloxera genotypes is an important aspect of management as genotypes differ in virulence and susceptibility to control using resistant rootstocks. Microsatellite markers developed on polyacrylamide gel systems have been the most widely used molecular method for phylloxera genotype identification, but this approach has been superseded by fluorescent capillary-based genotyping. The current study presents new laboratory methods for amplifying a standard set of eight phylloxera microsatellite markers using PCR-incorporated fluorescently labelled primers, genotyped on an ABI capillary platform. Comparison of allele size data scored on (i) polyacrylamide, (ii) capill

SUBMITTER: Blacket MJ 

PROVIDER: S-EPMC11856487 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature