Research on the Expression of Immune-Related Genes at Different Stages in the Third-Instar Larvae of Spodoptera frugiperda Infected by Metarhizium rileyi.

ABSTRACT: Spodoptera frugiperda is a major migratory agricultural pest that poses a significant threat to global crop safety. Metarhizium rileyi has emerged as an effective biocontrol agent against lepidopteran pests. In this study, we examined the immune responses of third-instar S. frugiperda larvae at various stages of an M. rileyi infection. Using RNA-seq and microscopic observation, we identified the immune-related pathways enriched at different infection stages, which were further validated by a qRT-PCR. Our findings revealed the following immune responses during infection: During the stage when M. rileyi penetrated the host cuticle (0-48 h), the genes related to energy metabolism, detoxification, and melanization were upregulated. Meanwhile, the TOLL and IMD

SUBMITTER: Xu P 

PROVIDER: S-EPMC11856804 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature