Two-Dimensional Chromatographic Isolation of High Purity Erinacine A from Hericium erinaceus.

ABSTRACT: A simple and robust two-dimensional chromatographic fractionation protocol for the isolation of the neuroprotective compound erinacine A from Hericium erinaceus is proposed. This production platform yielded 19.4 mg of erinacine A from approximately 130 g of mushroom material, with a chromatographic purity of 97.4%. The procedure includes extraction, concentration, fractionation, purification, and characterisation of the bioactive compound. The crude H. erinaceus extract was fractionated in the first dimension by normal-phase flash chromatography, and the fraction containing erinacine A was further purified in the second dimension by semi-preparative reversed-phase chromatography. This strategy utilises the orthogonality of the two chromatographic modes to effectively eliminat

SUBMITTER: Naumoska K 

PROVIDER: S-EPMC11856814 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature