Epigenetic Modulation Directs Recovery Post LASIK and SMILE Surgery: An Experimental Study.

ABSTRACT:

Purpose

refractive surgery, such as LASIK and SMILE, induces a wound healing response that leads to significant corneal stromal remodeling. We have shown that the protein profile in the stroma changes dramatically immediately post-surgery. However, the methylation status of the DNA post-refractive surgery remains unknown.

Design/participants

DNA methylation study. Refractive surgery (SMILE/LASIK) performed on donor eye globes.

Method

we investigated the epigenetic changes post-surgery in relation to long term ECM remodeling in an experimental ex vivo study design. Donor globes (n = 19) were obtained from the eye bank. Three globes served as non-surgical controls while SMILE (-6DS) and LASIK surgery (-6DS) were performed on eight globes each and incubated for 3 days an

SUBMITTER: Shetty R 

PROVIDER: S-EPMC11856829 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature