A VgrG2b fragment cleaved by caspase-11/4 promotes <i>Pseudomonas aeruginosa</i> infection through suppressing the NLRP3 inflammasome.

ABSTRACT: The T6SS of Pseudomonas aeruginosa plays an essential role in the establishment of chronic infections. Inflammasome-mediated inflammatory cytokines are crucial for host defense against bacterial infections. We found that P. aeruginosa infection activates the non-canonical inflammasome in macrophages, yet it inhibits the downstream activation of the NLRP3 inflammasome. The VgrG2b of P. aeruginosa is recognized and cleaved by caspase-11, generating a free C-terminal fragment. The VgrG2b C-terminus can bind to NLRP3, inhibiting the activation of the NLRP3 inflammasome by rejecting NEK7 binding to NLRP3. Administration of a specific peptide that inhibits caspase-11 cleavage of VgrG2b significantly improves mouse survival during infection. Our discovery elucidates a mechani

SUBMITTER: Qian Y 

PROVIDER: S-EPMC11856931 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature