Genomics-Driven Discovery of Plantariitin A, a New Lipopeptide in <i>Burkholderia plantarii</i> DSM9509.

ABSTRACT: A significant number of silent biosynthetic gene clusters (BGCs) within the Burkholderia genome remain uncharacterized, representing a valuable opportunity for the discovery of new natural products. In this research, the recombineering system ETh1h2e_yi23, which facilitates recombination in Burkholderia and was developed in our previous study, was used for mining the BGCs of B. plantarii DSM9509. By using this recombineering system, the constitutive promoter was precisely inserted into the genome, resulting in the activation of the silent pla BGC, which led to the production of a new lipopeptide named plantariitin A. A distinctive characteristic of this lipopeptide is the incorporation of a non-proteinogenic amino acid residue, i.e., amino-1,2,3,6-tetrahydro-2,6

SUBMITTER: Wang X 

PROVIDER: S-EPMC11858073 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature