In Vitro and In Silico Studies of Maculosin as a Melanogenesis and Tyrosinase Inhibitor.

ABSTRACT: The investigation of melanogenesis and tyrosinase inhibitors is essential for developing safe and effective natural compounds to treat pigmentation disorders. This study aimed to evaluate the effects of maculosin, a cyclic dipeptide composed of tyrosine and proline, on melanin production and tyrosinase activity using the B16F10 melanoma cell model, while elucidating its mechanism of action through molecular docking and molecular dynamics (MD) simulations. Experimental results demonstrated that maculosin inhibited intracellular melanin content and tyrosinase activity in a concentration-dependent manner in B16F10 melanoma cells. Molecular docking analyses revealed that maculosin exhibited high binding affinities with mushroom tyrosinase (mTYR), tyrosinase-related protein 1 (TYRP1), and Ba

SUBMITTER: Xu Y 

PROVIDER: S-EPMC11858074 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature