Metabolic Responses, Cell Recoverability, and Protein Signatures of Three Extremophiles: Sustained Life During Long-Term Subzero Incubations.

ABSTRACT: Few halophilic strains have been examined in detail for their culturability and metabolic activity at subzero temperatures, within the ice matrix, over the longer term. Here, we examine three Arctic strains with varied salinity tolerances: Colwellia psychrerythraea str. 34H (Cp34H), Psychrobacter sp. str. 7E (P7E), and Halomonas sp. str. 3E (H3E). As a proxy for biosignatures, we examine observable cells, metabolic activity, and recoverability on 12-month incubations at -5, -10 and -36 °C. To further develop life-detection strategies, we also study the short-term tracking of new protein synthesis on Cp34H at -5 °C for the first time, using isotopically labeled ¹³C₆-leucine and mass spectrometry-based proteomics. All three bacterial species remaine

SUBMITTER: Ewert M 

PROVIDER: S-EPMC11858272 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature