Adaptive Laboratory Evolution and Carbon/Nitrogen Imbalance Promote High-Yield Ammonia Release in Saccharomyces cerevisiae.

ABSTRACT: Ammonia, essential for fertilizers and energy storage, is mainly produced through the energy-demanding Haber-Bosch process. Microbial production offers a sustainable alternative, but natural yeast cells have not yet demonstrated success. This study aimed to enhance ammonia production in Saccharomyces cerevisiae by optimizing amino acid utilization through its deamination metabolism. Adaptive laboratory evolution is a method for rapidly generating desirable phenotypes through metabolic and transcriptional reorganization. We applied it to the efficiently fermenting S. cerevisiae strain CEN.PK113-7D using an unbalanced carbon/nitrogen medium to impose selective pressure. We selected several evolved strains with a 3-5-fold increase in amino acid utilization and ammonia secretion.

SUBMITTER: Pessina A 

PROVIDER: S-EPMC11858359 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature