Genetic Code Expansion for Controlled Surfactin Production in a High Cell-Density Bacillus subtilis Strain.

ABSTRACT:

Background

In biotechnology, B. subtilis is established for heterologous protein production. In addition, the species provides a variety of bioactive metabolites, including the non-ribosomally produced surfactin lipopeptide. However, to control the formation of the target product-forming enzyme, different expression systems could be introduced, including the principle of genetic code expansion by the incorporation of externally supplied non-canonical amino acids.

Methods

Integration of an amber stop codon into the srfA operon and additional chromosomal integration of an aminoacyl-tRNA synthetase/tRNA mutant pair from Methanococcus jannaschii enabled site-directed incorporation of the non-canonical amino acid O-methyl-L-tyrosine (OMeY). In different fed-b

SUBMITTER: Hermann A 

PROVIDER: S-EPMC11858380 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature