Screening and Optimization of Solid-State Fermentation for Esteya vermicola, an Entomopathogenic Fungus Against the Major Forest Pest Pine Wood Nematode.

ABSTRACT: Pine wilt disease (PWD), caused by the pine wood nematode (PWN, Bursaphelenchus xylophilus), is one of the most serious threats to pine forests worldwide. The fungus Esteya vermicola, with its lunate conidia capable of parasitizing the PWN, has shown promise as an efficient biological control agent against PWD. Solid-state fermentation (SSF) is preferred for large-scale applications in the field, as it facilitates microbial agent transport and ensures a long shelf life. However, research on enhancing the yield of lunate conidia from E. vermicola through SSF is limited. In this study, we initially achieved a yield of 3.04 × 10⁸ conidia/g using a basic SSF medium composed of wheat bran, corn flour, and soybean flour. To improve this yield, we employed an ortho

SUBMITTER: Zhang L 

PROVIDER: S-EPMC11858419 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature