Chemical Analysis of Plasma-Activated Culture Media by Ion Chromatography.

ABSTRACT: Background: Currently, the procedures and methods applied in biological and medical fields for the determination of reactive oxygen and nitrogen species (RONS), primarily rely on spectrophotometric techniques, which involve the use of colorimetric reagents. While these methods are widely accepted, they exhibit significant limitations from an analytical standpoint, particularly due to potential inaccuracies, artifacts, and pronounced susceptibility to matrix effects. The purpose of this Technical Note is to demonstrate the application of ion chromatography-a robust and well-established analytical technique-for the quantification of RONS produced in cell culture media through the exposure to cold atmospheric plasma (CAP), an innovative therapeutic approach for cancer treatment, known

SUBMITTER: Locatelli M 

PROVIDER: S-EPMC11858801 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature