An Improved Method for Agrobacterium-Mediated Genetic Transformation of Three Types of Lettuce.

ABSTRACT: Lettuce genetic transformation is genotype-dependent. In the present study, we have successfully developed an optimized Agrobacterium-mediated transformation protocol for elite lettuce cultivars, which belong to the romaine, leaf, and butterhead cultivar types. We optimized the type and concentration of plant growth regulators (PGRs) and selection antibiotics and found that the use of 1-naphthaleneacetic acid (NAA; 0.10 mg/L) and 6-benzyladenine (BA; 0.25 mg/L) as plant growth regulators, the use of hygromycin (15 mg/L) for transgenic plant selection, and the use of cotyledons and the first true leaf as explants efficiently yielded transformed plants for seven out of the eleven tested cultivars, achieving a 24.3-100% transformation efficiency. These seven cultivars include two romai

SUBMITTER: Roche MC 

PROVIDER: S-EPMC11858900 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature