Quality assessment of maize assembled genomic islands (MAGIs) and large-scale experimental verification of predicted genes.

ABSTRACT: Recent sequencing efforts have targeted the gene-rich regions of the maize (Zea mays L.) genome. We report the release of an improved assembly of maize assembled genomic islands (MAGIs). The 114,173 resulting contigs have been subjected to computational and physical quality assessments. Comparisons to the sequences of maize bacterial artificial chromosomes suggest that at least 97% (160 of 165) of MAGIs are correctly assembled. Because the rates at which junction-testing PCR primers for genomic survey sequences (90-92%) amplify genomic DNA are not significantly different from those of control primers ( approximately 91%), we conclude that a very high percentage of genic MAGIs accurately reflect the structure of the maize genome. EST alignments, ab initio gene prediction, and sequence simil

SUBMITTER: Fu Y 

PROVIDER: S-EPMC1186025 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature