Differential Gene Expression Analysis of Whole Blood Transcriptome Between Young and Old Border Collie Dogs.

ABSTRACT: Aging is the most significant risk factor for many diseases and increased mortality, and it is influenced by both genetic and environmental factors. In this study, our primary goal was to investigate age-related gene expression changes in whole blood samples collected from dogs and identify potential biomarkers of healthy aging. We sequenced the mRNA fraction of whole blood samples from five young and five old border collie dogs and performed differential gene expression and differential transcript usage analyses. The raw sequencing data exhibited high quality. Multidimensional scaling analysis failed to differentiate age clusters. Moreover, we identified only a limited number of differentially expressed genes (n = 61) and 30 genes with differential transcript usage between the blood trans

SUBMITTER: Jonas D 

PROVIDER: S-EPMC11860333 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature