Diagnostic Assays for Avian Influenza Virus Surveillance and Monitoring in Poultry.

ABSTRACT: Diagnostic testing plays a key role in a surveillance program as diagnostic testing aims to accurately determine the infection or disease status of an individual animal. Diagnostic assays for AIV can be categorized into four broad types: tests for detecting the virus, its antigen, its genomic material, and antibodies to the virus. Virus characterization almost always follows virus detection. The present article surveys the current literature on the goals, principles, test performance, advantages, and disadvantages of these diagnostic assays. Virus isolation can be achieved using embryonating eggs or cell cultures in a lab setting. Virus antigens can be detected by antigen-capturing immunoassays or tissue immunoassays. Viral RNA can be detected by PCR-based assays (gel-based reverse transcr

SUBMITTER: Azeem S 

PROVIDER: S-EPMC11860460 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature