Development of a Multiplex Conventional PCR Assay for Concurrent Detection of FAdV-4, FAdV-8b, and FAdV-11.

ABSTRACT: Fowl adenovirus (FAdV) belongs to the Aviadenovirus genus within the Adenoviridae family. FAdVs are widely distributed and associated with various diseases in poultry, including adenoviral gizzard erosion (AGE), hepatitis-hydropericardium syndrome (HHS), and inclusion body hepatitis (IBH). In this study, we developed a multiplex conventional PCR for simultaneously detecting FAdV-4, -8b, and -11 by targeting the hexon gene. The multiplex PCR was optimized for primer concentrations and thermocycling conditions. The optimal primer concentration combination was set at 0.125 μM for FAdV-4, -8b, and 0.25 μM for FAdV-11. Under these conditions, the limit of detection (LOD) was 10³ copies/μL of plasmid standards for FAdV-4, -8b, and -11. These results demonstrated that the

SUBMITTER: Kang SK 

PROVIDER: S-EPMC11860461 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature