Characterization and Protective Efficacy of a Salmonella Typhimurium ATCC 14028 sptP Mutant as a Live Attenuated Vaccine Candidate.

ABSTRACT:

Background

Salmonella Typhimurium poses a substantial health risk to both humans and animals. This study evaluated the potential of using the Salmonella Typhimurium ΔsptP mutant as a live-attenuated vaccine candidate by constructing it through homologous recombination and assessing its key biological properties, including growth characteristics, immunogenicity, and protective efficacy.

Methods

We generated the ΔsptP mutant through targeted gene deletion, ensuring the preservation of the bacterial strain's growth and stability. In vitro and in vivo assays were performed to compare the invasive capabilities between the mutant and the wild-type strains. Specifically, we examined the invasion into RAW264.7 murine macrophages and mice. Furthermore, the

SUBMITTER: Zhou N 

PROVIDER: S-EPMC11860608 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature